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Different authors mentioned that various species of yeasts, including Rhodoturula, have great benefits as immune promoters (Chen et al. 2019, Nakano & Wiegertjes 2020, Yun et al. 2021). According to Martin & Król (2017), the transcription of immune and stress-related genes is normally applied when investigating the impact of functional feed additives on aquatic animals to understand the mode of action on a gene basis. Cytokines (primarily generated by white blood cells) are particularly important and one of the most studied mediators of innate immunity response. Among them, we can mention interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), transforming growth-β (TGF-β), and heat shock protein 70 (HSP-70) (Chen et al. 2014, Wu et al. 2018). TNF-α and IL-1β play multiple functions, like the regulation of cell proliferation, are immune regulators and activate lymphocytes, macrophages, and natural killer cells, stimulating the release of other cytokines during the microbial invasion or when there is a tissue injury (Menaga et al. 2019, Lumsangkul et al. 2021). However, more research needs to be done in this regard. In fish, it has been shown that Hsp70 plays an essential role in immune defense against bacterial or viral infection, can stimulate the cytolytic activity of natural killer cells, and the production of NO and chemokines by macrophages and dendritic cells (Chen et al. 2014, Wu et al. 2018). The TGF-β superfamily plays active roles in immune cell functional regulation, cell proliferation, differentiation, and migration (Lin et al. 2014, Zhan et al. 2015). Transferrin is considered an activator of macrophages and an inhibitor of bacterial growth by sequestering the iron in the blood, limiting its availability for pathogens (Yin et al. 2018). This glycoprotein presents polymorphism, possibly as an adaptation to the capacity that they have developed pathogenic bacteria to obtain iron. Evidence indicates that some proteolytic fragments of transferrin can produce nitric oxide, which is also a powerful bactericidal agent (Stafford & Belosevic 2003). Our results agree with Wang et al. (2015) and Yang et al. (2015). They have mentioned that Rhodotorula sp. and R. benthica effectively colonized the gut of sea cucumbers and improved resistance against disease. R. mucilaginosa has been used as part of the diet with a mixture of microorganisms or alone and has been shown to promote innate immunity in species such as Atlantic salmon (Salmo salar), juvenile Nile tilapia, and rainbow trout (Oncorhynchus mykiss) (Chen et al. 2019, Wang et al. 2019, Schafberg et al. 2020).
In order to receive notification of the issuance of the EA and to keep track of all formal issuances and submittals in specific dockets, the Commission offers a free service called eSubscription. This can reduce the amount of time you spend researching proceedings by automatically providing you with notification of these filings, document summaries, and direct links to the documents. Go to www.ferc.gov/docs-filing/esubscription.asp. 59ce067264
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